Cambridge International AS & A Level BIOLOGY 9700/01 Paper 1 Multiple Choice For examination from 2022 SPECIMEN PAPER 1 hour 15 minutes You must answer on the multiple choice answer sheet. *0123456789* You will need: Multiple choice answer sheet Soft clean eraser Soft pencil (type B or HB is recommended) INSTRUCTIONS ● There are forty questions on this paper. Answer all questions. ● For each question there are four possible answers A , B , C and D . Choose the one you consider correct and record your choice in soft pencil on the multiple choice answer sheet. ● Follow the instructions on the multiple choice answer sheet. ● Write in soft pencil. ● Write your name, centre number and candidate number on the multiple choice answer sheet in the spaces provided unless this has been done for you. ● Do not use correction fluid. ● Do not write on any bar codes. ● You may use a calculator. INFORMATION ● The total mark for this paper is 40. ● Each correct answer will score one mark. ● Any rough working should be done on this question paper. This document has 18 pages. Blank pages are indicated. © UCLES 2019 [Turn over

Cambridge International AS & A Level
BIOLOGY 9700/01
Paper 1 Multiple Choice For examination from 2022
SPECIMEN PAPER 1 hour 15 minutes
You must answer on the multiple choice answer sheet.
*0123456789*
You will need: Multiple choice answer sheet
Soft clean eraser
Soft pencil (type B or HB is recommended)
INSTRUCTIONS
● There are forty questions on this paper. Answer all questions.
● For each question there are four possible answers A , B , C and D . Choose the one you consider correct
and record your choice in soft pencil on the multiple choice answer sheet.
● Follow the instructions on the multiple choice answer sheet.
● Write in soft pencil.
● Write your name, centre number and candidate number on the multiple choice answer sheet in the
spaces provided unless this has been done for you.
● Do not use correction fluid.
● Do not write on any bar codes.
● You may use a calculator.
INFORMATION
● The total mark for this paper is 40.
● Each correct answer will score one mark.
● Any rough working should be done on this question paper.
This document has 18 pages. Blank pages are indicated.
© UCLES 2019 [Turn over

2 1 Which definition of the magnification of a drawing of a leaf is correct? A the actual size of the leaf multiplied by the magnification of the microscope B the measured difference in size between the leaf and a drawing of the leaf C the increase in size of the leaf when observed using a microscope D the size of the drawing of the leaf divided by the actual size of the leaf 2 The electron micrograph shows part of two cells. W X Y Which labelled features identify these cells as eukaryotic? A W, X and Y B W and X only C W only D X only 3 Plant cells are stained and viewed using a student microscope. The light source is natural light. What could be clearly visible at u 400 magnification? A cristae of mitochondria B grana of chloroplasts C nucleoli D ribosomes © UCLES 2019 9700/01/SP/22

2
1 Which definition of the magnification of a drawing of a leaf is correct?
A the actual size of the leaf multiplied by the magnification of the microscope
B the measured difference in size between the leaf and a drawing of the leaf
C the increase in size of the leaf when observed using a microscope
D the size of the drawing of the leaf divided by the actual size of the leaf
2 The electron micrograph shows part of two cells.
W
X
Y
Which labelled features identify these cells as eukaryotic?
A W, X and Y B W and X only C W only D X only
3 Plant cells are stained and viewed using a student microscope. The light source is natural light.
What could be clearly visible at u 400 magnification?
A cristae of mitochondria
B grana of chloroplasts
C nucleoli
D ribosomes
© UCLES 2019 9700/01/SP/22

3 4 Which lengths are equivalent to 1 P m? 1 1000 mm 2 0.001 nm 3 0.001 mm 4 1 000 000 nm 5 0.01 mm 6 1000 nm A 1 and 4 B 2 and 5 C 3 and 4 D 3 and 6 5 Some secretory cells synthesise and release glycoproteins. What is the correct sequence of some of the events that occur in a secretory cell? 1 exocytosis 2 products accumulate in secretory vesicles 3 mRNA binds to ribosomes 4 synthesis of glycoproteins A 3, 4, 1, 2 B 3, 4, 2, 1 C 4, 3, 1, 2 D 4, 3, 2, 1 [Turn over © UCLES 2019 9700/01/SP/22

3
Which lengths are equivalent to 1 P m?
1000 mm
0.001 nm
0.001 mm
1 000 000 nm
0.01 mm
1000 nm
A 1 and 4 B 2 and 5 C 3 and 4 D 3 and 6
Some secretory cells synthesise and release glycoproteins.
What is the correct sequence of some of the events that occur in a secretory cell?
exocytosis
products accumulate in secretory vesicles
mRNA binds to ribosomes
synthesis of glycoproteins
A 3, 4, 1, 2 B 3, 4, 2, 1 C 4, 3, 1, 2 D 4, 3, 2, 1
© UCLES 2019 9700/01/SP/22 [Turn over

4 6 A scientist used a blender to break open a sample of animal cells and release the organelles. The extracted mixture was spun in a centrifuge at a low speed. The heaviest type of organelle sank to the bottom of the centrifuge tube to form a solid pellet, pellet 1. liquid above pellet solid pellet The liquid above pellet 1 was poured into a clean centrifuge tube and spun at a higher speed. The next heaviest organelle sank to the bottom to form a solid pellet, pellet 2. The scientist repeated this procedure twice more to obtain pellet 3 and pellet 4, each containing a single type of organelle. What is the function of the type of organelle extracted in pellet 3? A digestion of old cell organelles B production of ATP by respiration C synthesis of mRNA D synthesis of protein 7 What is the general formula for amylose? A (C H O ) B (C H O ) C (C H O ) D (C H O ) 5 10 5 n 5 10 6 n 6 12 6 n 6 10 5 n 8 Which statement describes how the molecular structure of starch is suited to its function? A Amylose has a branched structure and amylopectin is coiled to give a compact molecule for transport. B In the breakdown of amylose and amylopectin, many condensation reactions release stored energy. C In the formation of amylose and amylopectin, many hydrolysis reactions allow the release of stored energy. D Amylose and amylopectin form a complex that is insoluble and so does not affect the water potential of the cell. © UCLES 2019 9700/01/SP/22

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6 A scientist used a blender to break open a sample of animal cells and release the organelles.
The extracted mixture was spun in a centrifuge at a low speed. The heaviest type of organelle
sank to the bottom of the centrifuge tube to form a solid pellet, pellet 1.
liquid above
pellet
solid pellet
The liquid above pellet 1 was poured into a clean centrifuge tube and spun at a higher speed. The
next heaviest organelle sank to the bottom to form a solid pellet, pellet 2.
The scientist repeated this procedure twice more to obtain pellet 3 and pellet 4, each containing a
single type of organelle.
What is the function of the type of organelle extracted in pellet 3?
A digestion of old cell organelles
B production of ATP by respiration
C synthesis of mRNA
D synthesis of protein
7 What is the general formula for amylose?
A (C H O ) B (C H O ) C (C H O ) D (C H O )
5 10 5 n 5 10 6 n 6 12 6 n 6 10 5 n
8 Which statement describes how the molecular structure of starch is suited to its function?
A Amylose has a branched structure and amylopectin is coiled to give a compact molecule for
transport.
B In the breakdown of amylose and amylopectin, many condensation reactions release stored
energy.
C In the formation of amylose and amylopectin, many hydrolysis reactions allow the release of
stored energy.
D Amylose and amylopectin form a complex that is insoluble and so does not affect the water
potential of the cell.
© UCLES 2019 9700/01/SP/22

5 9 Cows and whales are mammals that produce milk to feed their babies. Newborn whales grow faster than newborn cows. Milk contains fatty acids with a range of chain lengths. The table shows the percentage of fatty acids of different chain lengths in cow milk and whale milk. chain length of fatty percentage of fatty acids in milk acids / number of cow whale carbon atoms 4–12 22.2 0.0 14 10.6 13.8 16 25.5 27.9 18 40.1 29.4 > 18 1.6 28.9 Which statement about the ratio of short fatty acids (4–16 carbon atoms) to long fatty acids (18 or more carbon atoms) in the milk of cows and whales is correct? A The ratio in cow milk is higher because newborn cows need more energy than newborn whales. B The ratio in cow milk is lower because newborn cows need less energy than newborn whales. C The ratio in whale milk is higher because newborn whales need less energy than newborn cows. D The ratio in whale milk is lower because newborn whales need more energy than newborn cows. 10 The structure of phospholipids includes: 1 polar phosphate heads 2 hydrophobic fatty acid chains 3 saturated fatty acid chains. What are essential for the formation of a phospholipid bilayer in a cell surface membrane? A 1 and 2 B 1 and 3 C 2 and 3 D 3 only [Turn over © UCLES 2019 9700/01/SP/22

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9 Cows and whales are mammals that produce milk to feed their babies. Newborn whales grow
faster than newborn cows. Milk contains fatty acids with a range of chain lengths.
The table shows the percentage of fatty acids of different chain lengths in cow milk and whale
milk.
chain length of fatty percentage of fatty acids in milk
acids / number of
cow whale
carbon atoms
4–12 22.2 0.0
14 10.6 13.8
16 25.5 27.9
18 40.1 29.4
> 18 1.6 28.9
Which statement about the ratio of short fatty acids (4–16 carbon atoms) to long fatty acids
(18 or more carbon atoms) in the milk of cows and whales is correct?
A The ratio in cow milk is higher because newborn cows need more energy than newborn
whales.
B The ratio in cow milk is lower because newborn cows need less energy than newborn whales.
C The ratio in whale milk is higher because newborn whales need less energy than newborn
cows.
D The ratio in whale milk is lower because newborn whales need more energy than newborn
cows.
10 The structure of phospholipids includes:
1 polar phosphate heads
2 hydrophobic fatty acid chains
3 saturated fatty acid chains.
What are essential for the formation of a phospholipid bilayer in a cell surface membrane?
A 1 and 2 B 1 and 3 C 2 and 3 D 3 only
© UCLES 2019 9700/01/SP/22 [Turn over

6 11 The diagram shows the molecular structure of a peptide. O NH 2 C CH H O CH 2 3 H H C N C C OH N C C N C H H H O CH H O 2 OH Which molecules would result from the complete hydrolysis of the peptide? O NH 2 C CH 2 CH H CH H H H 2 3 A N C COOH N C COOH N C COOH H H H H CH H 2 O NH OH 2 C CH H CH H H H 2 3 B N C COOH N C COOH N C COOH H H H H H C OH H CH O NH 3 2 C CH H CH H H H 2 3 C N C COOH N C COOH N C COOH H H H H CH H 2 OH O NH 2 C CH H H H H H 2 D N C COOH N C COOH N C COOH H H H H CH H 2 OH © UCLES 2019 9700/01/SP/22

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11 The diagram shows the molecular structure of a peptide.
O NH
2
C
CH H O CH
2 3
H
H C N C C OH
N C C N C
H H
H O CH H O
2
OH
Which molecules would result from the complete hydrolysis of the peptide?
O NH
2
C
CH
2
H CH H H H CH
2 3
A N C COOH N C COOH N C COOH
H H H
H CH H
2
O NH OH
2
C
H CH H H H CH
2 3
B N C COOH N C COOH N C COOH
H H H
H H C OH H
CH
O NH 3
2
C
H CH H H H CH
2 3
C N C COOH N C COOH N C COOH
H H H
H CH H
2
OH
O NH
2
C
H CH H H H H
2
D N C COOH N C COOH N C COOH
H H H
H CH H
2
OH
© UCLES 2019 9700/01/SP/22

7 12 The diagram shows a haemoglobin molecule. β -globin chain 3 α -globin chain 4 2 α -globin chain β -globin chain 1 Which row identifies the different parts of the molecule? 1 2 3 4 A D -helix E -pleated oxygen binding hydrophobic sheet site amino acids B oxygen binding hydrophilic D -helix hydrophobic site amino acids amino acids C haem group hydrophobic D -helix hydrophilic amino acids amino acids D hydrophobic E -pleated haem atom oxygen binding amino acids sheet site [Turn over © UCLES 2019 9700/01/SP/22

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12 The diagram shows a haemoglobin molecule.
β -globin chain
3
α -globin chain
4
2
α -globin chain
β -globin chain
1
Which row identifies the different parts of the molecule?
1 2 3 4
A D -helix E -pleated oxygen binding hydrophobic
sheet site amino acids
B oxygen binding hydrophilic D -helix hydrophobic
site amino acids amino acids
C haem group hydrophobic D -helix hydrophilic
amino acids amino acids
D hydrophobic E -pleated haem atom oxygen binding
amino acids sheet site
© UCLES 2019 9700/01/SP/22 [Turn over

8 13 A student investigated the effect of enzyme concentration on the rate of hydrolysis of protein in milk. When the enzyme and milk were mixed, the protein was hydrolysed and the mixture changed from cloudy to clear. The student investigated five different enzyme concentrations and recorded the time taken for the mixture to become clear. What is an appropriate control for this investigation? A Carrying out an experiment where the enzyme solution is replaced with water. B Carrying out each experiment in a thermostatically controlled water-bath at 35 °C. C Repeating each experiment three times for each of the five enzyme concentrations. D Using the same volume of enzyme solution for each of the five experiments. 14 What determines the specificity of an enzyme? 1 the covalent and other bonding between R groups of the polypeptide 2 the optimum pH of the enzyme 3 the covalent peptide bonds between amino acids of the polypeptide 4 the shape of the substrate molecule A 1, 2, 3 and 4 B 1 and 3 only C 1 only D 2, 3 and 4 only © UCLES 2019 9700/01/SP/22

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13 A student investigated the effect of enzyme concentration on the rate of hydrolysis of protein in
milk.
When the enzyme and milk were mixed, the protein was hydrolysed and the mixture changed
from cloudy to clear.
The student investigated five different enzyme concentrations and recorded the time taken for the
mixture to become clear.
What is an appropriate control for this investigation?
A Carrying out an experiment where the enzyme solution is replaced with water.
B Carrying out each experiment in a thermostatically controlled water-bath at 35 °C.
C Repeating each experiment three times for each of the five enzyme concentrations.
D Using the same volume of enzyme solution for each of the five experiments.
14 What determines the specificity of an enzyme?
1 the covalent and other bonding between R groups of the polypeptide
2 the optimum pH of the enzyme
3 the covalent peptide bonds between amino acids of the polypeptide
4 the shape of the substrate molecule
A 1, 2, 3 and 4 B 1 and 3 only C 1 only D 2, 3 and 4 only
© UCLES 2019 9700/01/SP/22

9 15 The graph shows the effect of temperature on the rate at which the enzyme in a biological washing powder digests and removes fruit juice stains. rate of reaction T temperature Which statements explain the shape of the graph at temperatures higher than T? 1 Bonds are broken between the R groups of the amino acids in the polypeptide chains of the enzyme. 2 There are more collisions between the enzyme and its substrate. 3 The tertiary structure of the enzyme is altered. 4 The shapes of the active site and the substrate are no longer complementary. A 1, 2 and 3 B 1, 2 and 4 C 1, 3 and 4 D 2, 3 and 4 16 Which statement describes carrier proteins in cell surface membranes? A Carrier proteins are found on the outer surface of the membranes allowing cell recognition. B Carrier proteins are involved in moving substances through the membranes by either active transport or passive transport. C Carrier proteins allow the binding of ligand molecules, which causes changes within cells. D Carrier proteins are involved in moving substances through the membranes by passive transport through water-filled pores. 17 What happens to a typical bacterium when it is placed in surroundings that have a higher water potential than the water potential inside the cell? A The bacterium bursts because the cell wall has no structural function. B The bacterium dies because water leaves the cell by osmosis. C There is no change because the cell wall is impermeable to water. D There is a net movement of water into the bacterium. [Turn over © UCLES 2019 9700/01/SP/22

9
15 The graph shows the effect of temperature on the rate at which the enzyme in a biological washing
powder digests and removes fruit juice stains.
rate of
reaction
T
temperature
Which statements explain the shape of the graph at temperatures higher than T?
1 Bonds are broken between the R groups of the amino acids in the polypeptide chains of
the enzyme.
2 There are more collisions between the enzyme and its substrate.
3 The tertiary structure of the enzyme is altered.
4 The shapes of the active site and the substrate are no longer complementary.
A 1, 2 and 3 B 1, 2 and 4 C 1, 3 and 4 D 2, 3 and 4
16 Which statement describes carrier proteins in cell surface membranes?
A Carrier proteins are found on the outer surface of the membranes allowing cell recognition.
B Carrier proteins are involved in moving substances through the membranes by either active
transport or passive transport.
C Carrier proteins allow the binding of ligand molecules, which causes changes within cells.
D Carrier proteins are involved in moving substances through the membranes by passive
transport through water-filled pores.
17 What happens to a typical bacterium when it is placed in surroundings that have a higher water
potential than the water potential inside the cell?
A The bacterium bursts because the cell wall has no structural function.
B The bacterium dies because water leaves the cell by osmosis.
C There is no change because the cell wall is impermeable to water.
D There is a net movement of water into the bacterium.
© UCLES 2019 9700/01/SP/22 [Turn over

10 18 By which process do hydrogencarbonate ions leave red blood cells? A active transport B endocytosis C facilitated diffusion D phagocytosis 19 In an experiment, fluorescent dyes were attached to proteins on the outer surface of cell surface membranes. Fluorescent dyes of one colour were attached to proteins of a living human cell and fluorescent dyes of a different colour were attached to proteins of a living mouse cell. The human cell and the mouse cell were then fused to form a hybrid cell. At first, the proteins attached to different fluorescent dyes remained separate, but after 40 minutes the proteins were distributed randomly across the hybrid cell surface membrane. What does this experiment show? A Proteins are found only on the outer surface of cell surface membranes. B Proteins in the outer layer of a bilayer do not penetrate into the inner layer. C Proteins move freely in the phospholipids of a bilayer. D The cell surface membranes of the two cells are bilayers. 20 The diagram shows the mitotic cell cycle. During which phase is DNA replicated? cytokinesis D C B A © UCLES 2019 9700/01/SP/22

10
18 By which process do hydrogencarbonate ions leave red blood cells?
A active transport
B endocytosis
C facilitated diffusion
D phagocytosis
19 In an experiment, fluorescent dyes were attached to proteins on the outer surface of cell surface
membranes. Fluorescent dyes of one colour were attached to proteins of a living human cell and
fluorescent dyes of a different colour were attached to proteins of a living mouse cell.
The human cell and the mouse cell were then fused to form a hybrid cell.
At first, the proteins attached to different fluorescent dyes remained separate, but after 40 minutes
the proteins were distributed randomly across the hybrid cell surface membrane.
What does this experiment show?
A Proteins are found only on the outer surface of cell surface membranes.
B Proteins in the outer layer of a bilayer do not penetrate into the inner layer.
C Proteins move freely in the phospholipids of a bilayer.
D The cell surface membranes of the two cells are bilayers.
20 The diagram shows the mitotic cell cycle.
During which phase is DNA replicated?
cytokinesis
D
C
B
A
© UCLES 2019 9700/01/SP/22

11 21 Bacterial cells divide by a process called binary fission. Which macromolecules must be synthesised for binary fission? 1 cell membrane proteins and RNA 2 DNA and peptidoglycan 3 enzymes and cellulose A 1, 2 and 3 B 1 and 2 only C 2 and 3 only D 3 only 22 The electron micrograph shows a group of human chromosomes. W Y X Which label is correct for each of the structures labelled W, X and Y? W X Y A centriole centromere chromatid B centriole centromere microtubule C centromere telomere chromatid D centromere telomere microtubule 23 Which statement about the behaviour of chromosomes during mitosis is correct? A They attach to the spindle fibres to contain them within the nucleus. B They condense to prevent further translation of genes. C They reach the poles of the cell and begin to uncoil. D They replicate to produce sufficient DNA to form two new nuclei. [Turn over © UCLES 2019 9700/01/SP/22

11
21 Bacterial cells divide by a process called binary fission.
Which macromolecules must be synthesised for binary fission?
1 cell membrane proteins and RNA
2 DNA and peptidoglycan
3 enzymes and cellulose
A 1, 2 and 3 B 1 and 2 only C 2 and 3 only D 3 only
22 The electron micrograph shows a group of human chromosomes.
W
Y
X
Which label is correct for each of the structures labelled W, X and Y?
W X Y
A centriole centromere chromatid
B centriole centromere microtubule
C centromere telomere chromatid
D centromere telomere microtubule
23 Which statement about the behaviour of chromosomes during mitosis is correct?
A They attach to the spindle fibres to contain them within the nucleus.
B They condense to prevent further translation of genes.
C They reach the poles of the cell and begin to uncoil.
D They replicate to produce sufficient DNA to form two new nuclei.
© UCLES 2019 9700/01/SP/22 [Turn over

12 24 Gene mutations can occur by substitution, deletion or insertion. What is the smallest part of a DNA molecule that can be changed by a gene mutation? A base B codon C gene D nucleotide 25 Which statements about mRNA are correct? 1 mRNA can form base pairs. 2 mRNA can form hydrogen bonds. 3 mRNA is single stranded. A 1, 2 and 3 B 1 and 2 only C 1 and 3 only D 2 and 3 only © UCLES 2019 9700/01/SP/22

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24 Gene mutations can occur by substitution, deletion or insertion.
What is the smallest part of a DNA molecule that can be changed by a gene mutation?
A base
B codon
C gene
D nucleotide
25 Which statements about mRNA are correct?
1 mRNA can form base pairs.
2 mRNA can form hydrogen bonds.
3 mRNA is single stranded.
A 1, 2 and 3 B 1 and 2 only C 1 and 3 only D 2 and 3 only
© UCLES 2019 9700/01/SP/22

13 26 The table shows the role of four different proteins involved in DNA replication. protein helicase topoisomerase single-strand DNA polymerase binding protein role unwinds the breaks and rejoins binds to separated synthesises strand parental DNA the DNA strands DNA strands to of DNA double helix stabilise them Which row shows the functions of these proteins? helicase topoisomerase single-strand DNA polymerase binding protein A adds DNA prevents original enables tension makes strands nucleotides to the strands re-forming caused by available as 3 c end of a growing complementary unwinding to be templates polynucleotide base pairs released strand B enables tension prevents original makes strands adds DNA caused by strands re-forming available as nucleotides to the unwinding to be complementary templates 3 c end of a growing released base pairs polynucleotide strand C enables tension makes strands adds DNA prevents original caused by available as nucleotides to the strands re-forming unwinding to be templates 3 c end of a growing complementary released polynucleotide base pairs strand D makes strands enables tension prevents original adds DNA available as caused by strands re-forming nucleotides to the templates unwinding to be complementary 3 c end of a growing released base pairs polynucleotide strand 27 Which type of sugar and which type of bond are found in a DNA molecule? type of sugar type of bond A disaccharide glycosidic B disaccharide phosphodiester C monosaccharide peptide D monosaccharide hydrogen [Turn over © UCLES 2019 9700/01/SP/22

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26 The table shows the role of four different proteins involved in DNA replication.
protein helicase topoisomerase single-strand DNA polymerase
binding protein
role unwinds the breaks and rejoins binds to separated synthesises strand
parental DNA the DNA strands DNA strands to of DNA
double helix stabilise them
Which row shows the functions of these proteins?
helicase topoisomerase single-strand DNA polymerase
binding protein
A adds DNA prevents original enables tension makes strands
nucleotides to the strands re-forming caused by available as
3 c end of a growing complementary unwinding to be templates
polynucleotide base pairs released
strand
B enables tension prevents original makes strands adds DNA
caused by strands re-forming available as nucleotides to the
unwinding to be complementary templates 3 c end of a growing
released base pairs polynucleotide
strand
C enables tension makes strands adds DNA prevents original
caused by available as nucleotides to the strands re-forming
unwinding to be templates 3 c end of a growing complementary
released polynucleotide base pairs
strand
D makes strands enables tension prevents original adds DNA
available as caused by strands re-forming nucleotides to the
templates unwinding to be complementary 3 c end of a growing
released base pairs polynucleotide
strand
27 Which type of sugar and which type of bond are found in a DNA molecule?
type of sugar type of bond
A disaccharide glycosidic
B disaccharide phosphodiester
C monosaccharide peptide
D monosaccharide hydrogen
© UCLES 2019 9700/01/SP/22 [Turn over

14 28 The diagrams represent transverse sections of three plant organs. 2 4 5 6 1 3 root stem leaf Which row is correct for phloem? root stem leaf A 1 3 5 B 1 4 6 C 2 3 6 D 2 4 5 29 Sucrose moves from a phloem sieve tube element into a root cell. Which changes to the water potential and the volume of liquid in the phloem sieve tube element are correct? water potential volume of liquid A becomes higher decreases B becomes higher increases C becomes lower decreases D becomes lower increases 30 Which statements about water movement in plants are correct? 1 Water can pass through cell walls containing layers of cellulose fibres. 2 Water can pass through cell walls containing rings or spirals of lignin. 3 Water cannot pass through a band of suberin in cell walls. A 1, 2 and 3 B 1 and 2 only C 1 and 3 only D 2 and 3 only © UCLES 2019 9700/01/SP/22

14
28 The diagrams represent transverse sections of three plant organs.
2
4
5
6
1 3
root stem leaf
Which row is correct for phloem?
root stem leaf
A 1 3 5
B 1 4 6
C 2 3 6
D 2 4 5
29 Sucrose moves from a phloem sieve tube element into a root cell.
Which changes to the water potential and the volume of liquid in the phloem sieve tube element
are correct?
water potential volume of liquid
A becomes higher decreases
B becomes higher increases
C becomes lower decreases
D becomes lower increases
30 Which statements about water movement in plants are correct?
1 Water can pass through cell walls containing layers of cellulose fibres.
2 Water can pass through cell walls containing rings or spirals of lignin.
3 Water cannot pass through a band of suberin in cell walls.
A 1, 2 and 3 B 1 and 2 only C 1 and 3 only D 2 and 3 only
© UCLES 2019 9700/01/SP/22

15 31 The photomicrograph is a transverse section of a leaf. 5 4 1 3 2 Which labelled features are characteristic adaptations of many xerophytes? A 1, 3, 4 and 5 B 1, 2 and 3 C 1, 2 and 5 D 2, 3, 4 and 5 32 A maize crop is growing successfully in a field in which the water potential of the soil is –40 kPa. What is the most likely water potential of the cell sap in the root hair cell? A –60 kPa B –40 kPa C –20 kPa D 0 kPa 33 The contraction of the heart is coordinated through electrical impulses passing through the cardiac muscle. What is the correct order of part of the sequence of these impulses? A right and left atria → sinoatrial node → atrioventricular node → ventricular walls B sinoatrial node → right and left atria → atrioventricular node → Purkyne tissue C sinoatrial node → right and left atria → Purkyne tissue → atrioventricular node D right and left atria → sinoatrial node → Purkyne tissue → ventricular walls 34 Which features allow the aorta to withstand high blood pressures? A collagen fibres and elastin fibres B collagen fibres and smooth muscle C elastin fibres and endothelium D endothelium and smooth muscle [Turn over © UCLES 2019 9700/01/SP/22

15
31 The photomicrograph is a transverse section of a leaf.
5
4
1
3 2
Which labelled features are characteristic adaptations of many xerophytes?
A 1, 3, 4 and 5 B 1, 2 and 3 C 1, 2 and 5 D 2, 3, 4 and 5
32 A maize crop is growing successfully in a field in which the water potential of the soil is –40 kPa.
What is the most likely water potential of the cell sap in the root hair cell?
A –60 kPa B –40 kPa C –20 kPa D 0 kPa
33 The contraction of the heart is coordinated through electrical impulses passing through the cardiac
muscle.
What is the correct order of part of the sequence of these impulses?
A right and left atria → sinoatrial node → atrioventricular node → ventricular walls
B sinoatrial node → right and left atria → atrioventricular node → Purkyne tissue
C sinoatrial node → right and left atria → Purkyne tissue → atrioventricular node
D right and left atria → sinoatrial node → Purkyne tissue → ventricular walls
34 Which features allow the aorta to withstand high blood pressures?
A collagen fibres and elastin fibres
B collagen fibres and smooth muscle
C elastin fibres and endothelium
D endothelium and smooth muscle
© UCLES 2019 9700/01/SP/22 [Turn over

16 35 Which row is correct for the pulmonary artery? blood carried thickness of wall lumen size A oxygenated thin large B oxygenated thick small C deoxygenated thin large D deoxygenated thick small 36 The graph shows oxygen dissociation curves of adult haemoglobin in different carbon dioxide concentrations, 1 and 2. 100 1 2 80 percentage 60 saturation of haemoglobin 40 with oxygen 20 0 0 2 4 6 8 10 12 14 16 partial pressure of oxygen / kPa Which conditions could change the shape of curve 1 to the shape of curve 2? A increased carbon dioxide concentration causing a decrease in pH B increased carbon dioxide concentration causing an increase in pH C decreased carbon dioxide concentration causing a decrease in pH D decreased carbon dioxide concentration causing an increase in pH 37 What maintains the necessary concentration gradients for carbon dioxide and oxygen in the lungs? A good ventilation of the lungs B large surface area of the alveoli C the capillaries are close to the walls of the alveoli D the walls of the alveoli are thin © UCLES 2019 9700/01/SP/22

16
35 Which row is correct for the pulmonary artery?
blood carried thickness of wall lumen size
A oxygenated thin large
B oxygenated thick small
C deoxygenated thin large
D deoxygenated thick small
36 The graph shows oxygen dissociation curves of adult haemoglobin in different carbon dioxide
concentrations, 1 and 2.
100 1
2
80
percentage
60
saturation of
haemoglobin
40
with oxygen
20
0
0 2 4 6 8 10 12 14 16
partial pressure of oxygen / kPa
Which conditions could change the shape of curve 1 to the shape of curve 2?
A increased carbon dioxide concentration causing a decrease in pH
B increased carbon dioxide concentration causing an increase in pH
C decreased carbon dioxide concentration causing a decrease in pH
D decreased carbon dioxide concentration causing an increase in pH
37 What maintains the necessary concentration gradients for carbon dioxide and oxygen in the
lungs?
A good ventilation of the lungs
B large surface area of the alveoli
C the capillaries are close to the walls of the alveoli
D the walls of the alveoli are thin
© UCLES 2019 9700/01/SP/22

17 38 Which cells and tissues are present in the trachea? ciliated epithelium cartilage A B D C goblet cells 39 The global mortality figures for some diseases in 2013 are shown in the table. cause of death millions of deaths HIV / AIDS 1.50 TB 1.40 malaria 1.03 cholera 0.02 How many millions of people died in 2013 from the bacterial diseases listed in the table? A 0.02 B 1.42 C 2.45 D 2.90 40 Why is it difficult for B-lymphocytes and T-lymphocytes to respond to the antigens on pathogens that are intracellular parasites? A The antigens are constantly mutating. B The antigens can destroy the lymphocytes. C The lymphocytes do not encounter the antigens. D The lymphocytes do not recognise the antigens. © UCLES 2019 9700/01/SP/22

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38 Which cells and tissues are present in the trachea?
cartilage ciliated epithelium
A
B
D C
goblet cells
39 The global mortality figures for some diseases in 2013 are shown in the table.
cause of death millions of
deaths
HIV / AIDS 1.50
TB 1.40
malaria 1.03
cholera 0.02
How many millions of people died in 2013 from the bacterial diseases listed in the table?
A 0.02 B 1.42 C 2.45 D 2.90
40 Why is it difficult for B-lymphocytes and T-lymphocytes to respond to the antigens on pathogens
that are intracellular parasites?
A The antigens are constantly mutating.
B The antigens can destroy the lymphocytes.
C The lymphocytes do not encounter the antigens.
D The lymphocytes do not recognise the antigens.
© UCLES 2019 9700/01/SP/22

18 BLANK PAGE Copyright Acknowledgements: Question 2 © B060/0031; MARILYN SCHALLER/SCIENCE PHOTO LIBRARY; TEM of the junction of two leaf cells ; www.sciencephoto.com Question 22 © P656/0023; BIOPHOTO ASSOCIATES/SCIENCE PHOTO LIBRARY; False-colour SEM of a group of human chromosomes ; www.sciencephoto.com Question 31 © C011/5248; DR KEITH WHEELER/SCIENCE PHOTO LIBRARY; Oleander leaf, light micrograph ; www.sciencephoto.com Permission to reproduce items where third-party owned material protected by copyright is included has been sought and cleared where possible. Every reasonable effort has been made by the publisher (UCLES) to trace copyright holders, but if any items requiring clearance have unwittingly been included, the publisher will be pleased to make amends at the earliest possible opportunity. Cambridge Assessment International Education is part of the Cambridge Assessment Group. Cambridge Assessment is the brand name of the University of Cambridge Local Examinations Syndicate (UCLES), which itself is a department of the University of Cambridge. © UCLES 2019 9700/01/SP/22

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BLANK PAGE
Copyright Acknowledgements:
Question 2 © B060/0031; MARILYN SCHALLER/SCIENCE PHOTO LIBRARY; TEM of the junction of two leaf cells ; www.sciencephoto.com
Question 22 © P656/0023; BIOPHOTO ASSOCIATES/SCIENCE PHOTO LIBRARY; False-colour SEM of a group of human chromosomes ;
www.sciencephoto.com
Question 31 © C011/5248; DR KEITH WHEELER/SCIENCE PHOTO LIBRARY; Oleander leaf, light micrograph ; www.sciencephoto.com
Permission to reproduce items where third-party owned material protected by copyright is included has been sought and cleared where possible. Every
reasonable effort has been made by the publisher (UCLES) to trace copyright holders, but if any items requiring clearance have unwittingly been included, the
publisher will be pleased to make amends at the earliest possible opportunity.
Cambridge Assessment International Education is part of the Cambridge Assessment Group. Cambridge Assessment is the brand name of the University of
Cambridge Local Examinations Syndicate (UCLES), which itself is a department of the University of Cambridge.
© UCLES 2019 9700/01/SP/22

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